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KMID : 0364820210570020125
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Korean Journal of Microbiology
2021 Volume.57 No. 2 p.125 ~ p.128
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Draft genome sequence of bacterial strain BA299 in the genus Alkalihalobacillus, isolated from soil of a solar saltern in Korea
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Yoon Ki-Hong
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Abstract
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A bacterial strain BA299, belonging to the genus Alkalihalobacillus,was isolated from a saltern soil sampled at Anmyeon Islandlocated in Taean, South Korea. The draft genome sequence ofBA299 was determined using Illumina MiSeq platform. Thegenome comprises 69 contigs with genome size of 5,137,563bp long and GC content of 36.7%. The draft genome contains4,844 protein-coding genes, 106 pseudogenes and 160 RNAgenes including 34 ribosomal RNA genes, 119 transfer RNA(tRNA) genes and 7 non-coding RNA (ncRNA) genes. Thephylogenomic analysis based on the whole genome sequencedemonstrated that strain BA299 formed a distinct phyletic linefrom Alkalihalobacillus alkalinitrilicus DSM 22532T showingaverage nucleotide identity (ANI) and digital DNA?DNAhybridization (dDDH) values of 80.5 and 25.8%, respectively.
The strain BA299 contains exclusively a hydantoin racemasegene from other members of the genus Alkalihalobacillus,which is involved in the production of optically pure aminoacids.
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KEYWORD
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Alkalihalobacillus, BA299, draft genome sequence, hydantoin racemase, Illumina MiSeq
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